Physics & Astronomy Dept. Equipment

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The new hire will interact with data collected from the Lidke and Chakraborty labs (among others) and we list
here the relevant data generating microscopy equipment.

Lidke Labs

  • A custom assembled total internal reflectance fluorescence (TIRF) microscope setup: Olympus IX71
    inverted microscope, Andor iXon 897 EMCCD, 3D piezo nano-stage and four excitation lasers: 405 nm
    (15 mW), 488 nm (100 mW), 561 nm (100 mW) and 635 nm (100 mW).
  • A custom built high-speed hyperspectral microscope: Olympus IX71 inverted microscope, Andor Zyla 4.2+, 3D piezo nano-stage, 488 nm laser (40 mW) and custom built spectrometer.
  • A high-speed, two-color SPT tracking system: Olympus IX71 inverted microscope, 2X Andor iXon 860 EMCCDs, and laser excitation sources 532 nm (200 mW) 638 nm (100 mW).
  • A general prototyping microscope: MadCity Labs piezo objective positioner, 3D piezo nano-stage, Andor Luca EMCCD, dedicated laser excitation (others brought with fiber) of 405 nm (50 mW), 635 nm (25 mW) and custom optics.
  • A custom, high-speed 3D epi-fluorescence microscope: 3 axis positioner with z-axis piezo stage, Hamamatsu Orca Flash 4.0 sCMOS camera, 647 nm excitation (500 mW), and custom scanning optics.
  • A custom-built Raman imaging light-sheet microscope: Custom dual-objective design with 3.74mm WD, 0.67 N.A., 28.6x water objective (Special Optics) for excitation and 1.43-2.04 mm WD, 1.1 N.A., 25x apochromat water immersion objective (Nikon) for detection. Hamamatsu ORCA-Fusion Gen-III sCMOS camera, 785 nm (70mW) and 635 nm (5mW) laser excitation, Nano-F200W piezo objective lens positioner, galvanometer mirror scanning, custom built spectrometer (in development).

The following objectives are shared between microscope setups: 150X 1.45 NA TIRF, 100X 1.48 NA TIRF, 60X 1.4 NA Oil, two 60X 1.2 NA Water, 1.3 NA Si. Microscope setups are located across several compressed-air isolated optical tables: two 3’x5’, two 3'x4', and one 4'x8'. This lab also contains a work bench and sink, refrigerator, and several sets of pipetters.

Chakraborty Lab

The lab houses four 8’x6’ vibration isolation-tables along with several work-benches. On two of these isolation-tables different variants of lightsheet microscopes are already being developed. The lab has several laser sources: (1) Coherent OBIS (488, 561, 637 nm) 150mW housed in a 5 bay laser box with Power Supply and (2) Sapphire 488-400 LPX CDRH Laser System. There is a Hamamatsu sCMOS camera that is being shared between the two setups. There are two high end Dell workstations in the lab which are designated for instrument control through FPGA boards and breakout-boxes.

The lab contains refrigerators, vortexer, desktop centrifuge, and 37 C plate warmers, chemical storage cabinets and other laboratory supplies. 2x Cat 6a ethernet cables are wired from each table (through overhead ceiling) to a server rack in the spine containing Gbit switches. The server rack will be connected with cat 6a cable to the building ethernet and an optical fiber to the PAIS server room, which itself has a 40 Gbps connection to the UNM Center for Advanced Research Computing (CARC). Further storage and compute capabilities are available through CARC. In order to carry out rapid prototyping the lab is also equipped with a high quality 3D printer (Raise3D Pro2) with dual extruder and sub-100um resolution PLA print capability. 3D parts are designed using Adobe Inventor and then printed in the lab before sending to the machine shop for CNC machining.